本产品是根据本公司的双染料 RT-LAMP 专利技术开发的荧光和可见光双模式检测试剂盒,它既可以用于荧光 RT-LAMP 扩增及检测,也可以用于可视化 RT-LAMP扩增及检测,适用于各种针对核酸的快速定性检测。本产品具有下列特点:
1. 含可见光和荧光染料,既可以用金属浴和水浴扩增用可见光肉眼判断结果,也可用荧光 PCR 仪进行实时荧光检测。
2. 内含的 dUTP-UNG 可防交叉污染。
3. 特异性比 RT-PCR 高,因为 RT-LAMP 扩增使用 4-6 条引物而不是两条。
4. 灵敏性可达 10 拷贝/反应以下(随引物而不同),故假阴性率更低。
只可用于科研,只可进行定性检测,不能用于定量检测。
It can be used for both fluorescent RT-LAMP amplification and visual RT-LAMP amplification and detection, and is suitable for various rapid qualitative assays for nucleic acids. This product has the following features.
1. contains visible light and fluorescent dyes, can be used for both metal bath and water bath amplification with visible light to determine the results with the naked eye, and can also be used for real-time fluorescence detection with a fluorescent PCR instrument.
2. dUTP-UNG is included to prevent cross-contamination. 3.
3. higher specificity than RT-PCR, because RT-LAMP amplification uses 4-6 primers instead of two. 4.
4. sensitivity can be up to 10 copies/reaction (varies with primers), so the false negative rate is lower.
5. Can only be used for scientific research, only for qualitative testing, not for quantitative testing.